Part:BBa_K1510001:Design

Truncated sequence from BBa_K523013 that this part refers to a constitutive promoter and a RBS.

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 10
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NotI site found at 1
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 10
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 10
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

In order to let E.coli constitutively express the coding sequence, we search for plenty choices of constituve promoter. Eventually, we chose this truncated sequence of part BBa_K523013 as our upstream promoter and RBS sequence.

Source

This truncated sequence come from primer PCR from part BBa_K523013.

Reference: https://parts.igem.org/Part:BBa_K523013